Description
The test principle applied in this kit is enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Instant Androstenedione (ASD). Standards or samples and HRP-labeled detection antibody specific to Instant Androstenedione (ASD) (Detection Reagent A) are then added to the appropriate microtiter plate wells. Next, TMB substrate solution is added, only those wells that contain Instant Androstenedione (ASD), and HRP-labeled detection antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Instant Androstenedione (ASD) in the samples is then determined by comparing the O.D. of the samples to the standard curve